منابع مشابه
Changing the ligand specificity of CooA, a highly specific heme-based CO sensor.
The CO-specific heme-based sensor CooA regulates the ability of Rhodospirillum rubrum to grow on CO as an energy source. Only CO triggers the conformational change of CooA essential for the protein to function as a transcriptional activator. A structurally informed mutagenesis, followed by an in vivo screening method, allowed the isolation of a series of novel CooA variants that show very subst...
متن کاملFunctionally critical elements of CooA-related CO sensors.
CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow on CO as a sole energy source. We have identified a number of CooA homologs through database searches, expressed these heterologously in Escherichia coli, and monitored their ability to respond to CO in vivo. Further in vitro analysis of two CooA homologs from Azotobacter vinelandii and Carb...
متن کاملEffect of DNA binding on geminate CO recombination kinetics in CO-sensing transcription factor CooA.
Carbon monoxide oxidation activator (CooA) proteins are heme-based CO-sensing transcription factors. Here we study the ultrafast dynamics of geminate CO rebinding in two CooA homologues, Rhodospirillum rubrum (RrCooA) and Carboxydothermus hydrogenoformans (ChCooA). The effects of DNA binding and the truncation of the DNA-binding domain on the CO geminate recombination kinetics were specifically...
متن کاملThe Effects of Nano In2O3 and ZnO on the CO Gas Detection of the SnO2 Sensor
The pellet-type SnO2 sensor was synthesized by the solid state method and the effects of additives such as nano ZnO (1-12 mol %) and nano In2O3 (1-10 mol %) on the CO gas sensitivity of sensor were investigated. The optimum sintering temperature was chosen 800°C because of the porosity content of the samples. The phase analysis and microstructure of the samples were studied by x-ray diffraction...
متن کاملCooA, a CO-sensing transcription factor from Rhodospirillum rubrum, is a CO-binding heme protein.
Biological sensing of small molecules such as NO, O2, and CO is an important area of research; however, little is know about how CO is sensed biologically. The photosynthetic bacterium Rhodospirillum rubrum responds to CO by activating transcription of two operons that encode a CO-oxidizing system. A protein, CooA, has been identified as necessary for this response. CooA is a member of a family...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2003
ISSN: 0021-9258
DOI: 10.1074/jbc.m301000200